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Boris Negrutskii

Boris Negrutskii

AUFF-Ukraine research fellow, Aarhus Institute of Advanced Studies, Denmark and 

Professor, Department of Structural and Functional Proteomics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv, Ukraine

 

Research area

The structures of a human organism are mostly formed by proteins. Functioning of a human organism is mostly supported by proteins. Consequently, a proper amount of every protein should be maintained inside proper compartments of the cells at every time point of human life as even a slight disbalance could cause essential negative consequences. Mechanisms of protein synthesis in bacteria are known, however, despite similarity of principles, synthesis of mammalian proteins shows huge difference in the structural, functional and regulatory details that are still not known in full.

I study the proteins called translation elongation factors which are responsible for synthesis of all human proteins. An essential eukaryotic translation factor eEF1A contributes to the elongation of polypeptide chain by ribosome. It exists as two 97% homologous isoforms whose presence in mammalian cells is mutually exclusive and developmentally regulated. De novo mutations of A2 isoform cause severe neurological and heart diseases, this isoform is also markedly oncogenic. Despite significant medical and fundamental interest in the problem, the exact reason behind the different functionality of the two practically similar proteins is not known.

The project financed by the AUFF-Ukraine research fellowship is directed towards solving this puzzle at the level of primary functions of these proteins. Apart from being of independent scientific importance, this project nicely complements our job on resolving possible structural differences of the isoforms which is impossible to continue now in Ukraine because of the Russian invasion.

Short bio

I am an AUFF-Ukraine research fellow holding a Ph.D. in Molecular Biology from IMBG, Kyiv, Ukraine. My postdoctoral studies were at the UConn Health Center, Farmington, CT, USA and the Laboratory of Enzymology and Structural Biology of CNRS, Gif-sur-Yvette, France. Scientific achievements include a contribution to the discovery of channeling of aminoacyl-tRNA for mammalian protein synthesis, solving the X-ray structure of mammalian translation elongation factor eEF1A2, revealing the spatial organization of the human multiprotein nucleotide exchange complex eEF1B and related studies.

Collaborating department at Aarhus University during his fellowship

The Department of Molecular Biology and Genetics, Faculty of Natural Sciences, Aarhus University

Area of research:

Structural and functional proteomics    

Fellowship period:

22 April 2022 - 21 Oct 2022

Contact:

borisneg@aias.au.dk